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Cryo-FIB sample preparation
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Cryo-FIB sample preparation

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The molybdenum grids are mechanically hard with small expansion coefficient in the liquid nitrogen, which benefits the workflow of multi-step transfer in cryo-FIB experiments. The flat edge of the grid can keep the same direction of the grid between cryo-FIB milling and cryo-TEM, which is important for the following cryo-ET data collection.

There are two positions designed for the placement of cryo-grid boxes, ensuring a smooth transfer of cryo-grids from the cryo-grid box into the cryo-transfer shuttle. The shuttle position is designed for fixing the shuttle that is ready to install or recover the cryo-grids.

The transfer shuttle is made with pure copper material. The crosshead fixing screw is designed to lock the cover during the storage of the shutter after FIB milling in some cases.


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